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Western blotting against rP51, rGroEL, rNsp2, and rNsp3 using PHF-positive equine sera. Recombinant P51, rGroEL, rNsp2, and rNsp3 were separated by SDS-PAGE and probed with 1:500 dilution of PHF-positive horse sera (PHF sera, 1-15) and negative sera (Neg sera, N1-N3). Molecular masses are shown for each recombinant protein. Information regarding the sera samples is given in Table 4.
