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. 2001 Sep 15;15(18):2396–2407. doi: 10.1101/gad.918201

Figure 3.

Figure 3

N-terminal hCdc20 and hCdh1 inhibit APC in vitro. (A) In vitro degradation assay using Δ90 extracts or Cdh1 extracts of Xkid, cyc B, geminin, securin, and mB99 (Cdh1 extracts only) in the presence of buffer or 5 μM bacterially expressed H6Cdc20N120KENNA or H6Cdh1N125. Initial and 90 min time points are shown. Although we observe variability in half-lives between extracts, careful titration and kinetic analysis shows that half-lives are extended to ⋝60 min in the presence of 5 μM amounts of inhibitory N-terminal fragments.