Table 2.
Quantitative characterization of TOP10 bearing the HSL-inducible lysis device in low copy plasmid grown at 37°C in microplate
| TOP10 with pLC-T4LysHSL | |||
|---|---|---|---|
| Exponential phase | Early stationary phase | Late stationary phase | |
| Lysis entity [%] | 76.28 ± 0.3 | 75.43 ± 1.1 | 50.1 ± 2.5 |
| Lysis delay after induction [min] | 15 | 15 | 15 |
| Doubling time [min] | 49.8 ± 1.1 | ||
| Doubling time of negative control [min] | 43 ± 1.3 | ||
Induction was carried out with HSL 100 nM. Mean lysis entity and lysis delay after the induction are reported for the different growth phases together with their standard error, measured on 3 independent experiments. The doubling time of the uninduced lysis device and its negative control are reported too.