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Immunocytochemical analysis of CV-1 cells, transfected/infected either with SV40(776) WT or the Pt mutant genome, for detection of LT-Ag expression. CV-1 cells were transfected either with SV40(776) WT or the Pt mutant genome, fixed at 4th day posttransfection, incubated with a primary anti-SV40 LT-Ag (Ab-2) antibody followed by incubation with a FITC-conjugated goat anti-mouse secondary antibody and examined under a fluorescence microscope for detection of SV40 LT-Ag expression.
