Figure 3.
Immunocytochemical analysis of SVG-A cells transfected/infected either with JCV Mad-1 WT or its Pt mutant genome for detection of agnoprotein (AGNO) and VP1 expression. SVG-A cells were transfected either with JCV Mad-1 WT or the Pt mutant genome, fixed at 6th day posttranfection, incubated with a primary anti-Agno polyclonal and anti-VP1 (PAb597) monoclonal antibodies followed by incubation with a FITC-conjugated goat anti-rabbit and Rhodamine-conjugated goat anti-mouse secondary antibodies and examined under a fluorescence microscope for detection of JCV agnoprotein and VP1 expression. DAPI staining was also performed to visualize the nucleus of the cells.