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Transcripts of targeted viral genes produced by RT-PCR prior to insertion into plasmids. Capsid regions of NoV GI-5 (A), NoV GI-6 (B), NoV GII-4 (C), HRV VP7 (D), and HAV VP1/P2A (E). Lane 1, 100 bp ladder; lane 2, negative controls; lane 3, positive results.
