Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Sep 15;15(18):2421–2432. doi: 10.1101/gad.922801

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2001, Cold Spring Harbor Laboratory Press

PMC Copyright notice

JIP1 deficiency causes reduced oxygen and glucose deprivation-induced JNK activation and apoptosis of hippocampal neurons. (A,B) Wild-type (A) and Jip1^−/− (B) hippocampal neurons were cultured in vitro and visualized by MAP2 immunocytochemistry. (C,D) Activated JNK (red) was detected by immunofluorescence analysis of wild-type (C) and Jip1^−/− (D) hippocampal neurons following oxygen and glucose deprivation (OGD) challenge (4 h) and recovery (12 h) by staining with an antibody to phospho-(pThr¹⁸³pTyr¹⁸⁵)-JNK. Neurites were detected by staining with an antibody to MAP2 (green). A marked increase in the amount of activated JNK was observed in wild-type neurons (C), but not in Jip1^−/− neurons (D) following exposure to stress. (E,F) Cell death was measured by the percentage of TUNEL-positive nuclei (red) in the total number of bis-benzimide-stained nuclei (blue) following OGD challenge (4 h) and recovery (24 h). Increased numbers of apoptotic neurons were detected in wild-type (E) compared with Jip1^−/− (F) cultures. (G,H) The survival of wild-type (G) and Jip1^−/− (H) hippocampal neurons was compared by MAP2 immunocytochemistry following OGD challenge (4 h) and recovery (48 h). (I,J) Wild-type and Jip1^−/− hippocampal neurons were exposed to OGD challenge. Apoptosis measured by TUNEL assay (I) and survival measured by MAP2 staining (J) was quantitated in three independent experiments (mean ± SD). Scale bar in H, 200 μm (panels A,B,E–H) and 20 μm (panels C,D).

JIP1 deficiency causes reduced oxygen and glucose deprivation-induced JNK activation and apoptosis of hippocampal neurons. (A,B) Wild-type (A) and Jip1^−/− (B) hippocampal neurons were cultured in vitro and visualized by MAP2 immunocytochemistry. (C,D) Activated JNK (red) was detected by immunofluorescence analysis of wild-type (C) and Jip1^−/− (D) hippocampal neurons following oxygen and glucose deprivation (OGD) challenge (4 h) and recovery (12 h) by staining with an antibody to phospho-(pThr¹⁸³pTyr¹⁸⁵)-JNK. Neurites were detected by staining with an antibody to MAP2 (green). A marked increase in the amount of activated JNK was observed in wild-type neurons (C), but not in Jip1^−/− neurons (D) following exposure to stress. (E,F) Cell death was measured by the percentage of TUNEL-positive nuclei (red) in the total number of bis-benzimide-stained nuclei (blue) following OGD challenge (4 h) and recovery (24 h). Increased numbers of apoptotic neurons were detected in wild-type (E) compared with Jip1^−/− (F) cultures. (G,H) The survival of wild-type (G) and Jip1^−/− (H) hippocampal neurons was compared by MAP2 immunocytochemistry following OGD challenge (4 h) and recovery (48 h). (I,J) Wild-type and Jip1^−/− hippocampal neurons were exposed to OGD challenge. Apoptosis measured by TUNEL assay (I) and survival measured by MAP2 staining (J) was quantitated in three independent experiments (mean ± SD). Scale bar in H, 200 μm (panels A,B,E–H) and 20 μm (panels C,D).

HHS Vulnerability Disclosure