Table 1.

Kinetic parameters for ht-ADH variants at pH 7, 30 °C

Enzyme	kcat, s-1	Km (Alcohol), mM	Km (NAD+), mM	*
Wild type	24.9 (±2.5)	6.8 (±0.5)	1.1 (±0.1)	3.2 (±0.2)
L176V	1.7 (±0.1)	5.3 (±0.9)	1.2 (±0.2)	4.1 (±0.4)
L176A	2.8 (±0.3)	5.8 (±1.5)	2.6 (±0.6)	4.1 (±0.6)
L176G	7.7 (±1.4)	9.8 (±3.4)	25 (±8)	4.0 (±1.1)
L176Δ	0.43 (±0.09)	7.5 (±3.5)	37 (±14)	4.3 (±1.3)
V260A†	2.4 (±0.2)	4.2 (±0.8)	10 (±1.6)	4.1 (±0.7)

^*This is the ratio of k_cat measured with H-benzyl alcohol to that measured with D-benzyl alcohol.

^†The Arrhenius break for V260A is at approximately 40 °C; the rest are at 30 °C.