Fig. 2.

Characterization and multipotency of YFP⁺ cells in vitro. (A–I) Immunocharacterization of YFP⁺ cells before FACS: YFP⁺ cells (green) express the immature cell markers (red) Nestin (A–C), Sox9 (D–F), and PSA-NCAM (G–I). (J) Percentages of YFP⁺ cells expressing immature cell markers are maintained after short- and long-term culture compared with YFP⁺ cells at the time of FACS (mean ± SEM, n = 3 per group). (K) RT-PCR on short- and long-term FACS-sorted YFP⁺ spheres. Total RNA from whole adult spinal cord was used as control. (L–O) In NC differentiation medium, YFP⁺ cells differentiate into astrocytes (L), SC (M), neurons (N), and myofibroblasts (O).