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. 2011 Jun 13;108(26):10762–10767. doi: 10.1073/pnas.1018875108

Table 1.

Concentrations of NO3 and HTGs in leaf tissue under different stress conditions

Concentration of nitrate, mg/g d.w. Concentration of HTG [1]*, μg/g d.w. Concentration of HTG [2]*, μg/g d.w.
No days of treatment 4 7 4 7 4 7
Full nutrient supplied 49.6 ± 4.8 36.1 ± 1.5 n.d. n.d. n.d n.d
Total nutrient deprived 15.8 ± 1.0 3.6 ± 0.8 1155 ± 328 4729 ± 298 120 ± 61 1145 ± 298
Nitrate deprived 7.4 ± 2.4 0.2 ± 0.2 731 ± 240 2395 ± 366 383 ± 211 431 ± 152
Ammonium substituted 15.5 ± 4.4 2.7 ± 0.6 80 ± 41 2480 ± 218 n.d. 486 ± 56
Potassium deprived 32.6 ± 14.7 29.0 ± 1.9 44 ± 40 695 ± 170 n.d. 90 ± 57
Salt stress 36.3 ± 3.8 27.9 ± 2.4 n.d. n.d. n.d. n.d.
Oxidative stress 41.2 ± 6.2 12.9 ± 1.7 170 ± 75 2583 ± 518 21 ± 20 571 ± 137
Osmotic stress 61.6 ± 4.0 72.3 ± 10.4 n.d. n.d. n.d. n.d.
Root damage 23.0 ± 4.9 14.5 ± 2.7 119 ± 76 1920 ± 21 23 ± 21 529 ± 220
Leaf damage 39.1 ± 1.9 47.5 ± 11.5 n.d. n.d. n.d. n.d.
Cold treatment 49.9 ± 11.6 21.8 ± 5.8 0.25 ± 0.25 n.d. n.d. n.d.

*Values represent average (± SD) of three biological and two technical replicates (n = 6); integration was achieved by summation of the NMR regions: δ5.725–5.685 [1] and δ5.635–5.585 [2]. Where HTG concentration was <100 μg/g, values stated are considered to be less accurate and are given as indications of the presence of a small peak. However, in all cases the presence of the HTG was confirmed by the presence of the relevant methyl group singlet in NMR and the 287/303 ions in positive ion ESI. n.d., not detected.