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. 2011 Jun 13;108(26):10448–10453. doi: 10.1073/pnas.1106971108

Fig. 4.

Fig. 4.

BRC1, -2, -3, and -4 prevent formation of RAD51-dsDNA complexes. (A) EMSA analysis showing RAD51 (3 μM) incubated with GST-BRC1 prior to incubation with 32P-labeled dA40·dT40 dsDNA (0.3 μM, bp) and further incubation for 1 h in the presence of ATP, Mg2+, and Ca2+. Protein-DNA complexes were resolved in 6% PAGE and analyzed by autoradiography. Lane 1 contains DNA alone; lane 2 contains RAD51 incubated with DNA in the absence of BRC peptide. Lanes 3–8 contain the indicated concentration of BRC1 peptide and 3 μM RAD51. Lane 9 contains the maximum concentration of BRC1 peptide and DNA in the absence of RAD51. Lane 10 contains the indicated concentration of BRC4 and RAD51 incubated with DNA under the same conditions used with BRC1. (B) Same as in A but using BRC2 instead of BRC1. (C) Same as in A but using BRC3 instead of BRC1. (D) Quantification of data in A, B, and C: BRC1 (green), BRC2 (orange), BRC3 (blue), and BRC4 (purple; gel shown in Fig. S3). Error bars indicate SD from at least three independent experiments.