Table 2.
Apparent affinity of individual BRC repeat binding to the RAD51-ssDNA filament
| BRC repeat | Kd (μM) |
| BRC1 | N.A. |
| BRC2 | N.A. |
| BRC3 | N.A. |
| BRC4 | 14.6 ± 8.9 |
| BRC5 | 0.2 ± 0.1 |
| BRC6 | 2.2 ± 0.6 |
| BRC7 | 2.2 ± 1.1 |
| BRC8 | 5.8 ± 1.8 |
The apparent affinity of the BRC repeats for RAD51-ssDNA nucleoprotein filaments is defined as the concentration of BRCx required for half-maximal RAD51 nucleoprotein filament formation. The apparent dissociation constant (Kd,app ± S.D.) for BRCx binding was obtained from fitting of the EMSA experiments shown in Fig. 3 E and F. For the data that could not be fit reliably, no values are listed (N.A.).