Fig. 4.

Requirement for Rsc4 acetylation in the context of replication stress. (A) The double-mutant rsc4K25R nhp10Δ has delayed S-phase progression in MMS. Shown are representative cytometry profiles (n = 3 experiments). N, DNA copy number. (B) Representative profiles for midlog (asynchronous) cultures used in A. (C) Budding indices for cells treated as in A (n = 3; error bars indicate SDs). Cells with a bud diameter <50% of that of the mother cell were scored as small-budded cells, and cells with a diameter >50% of that of the mother cell were scored as large-budded cells. (D) Rsc4 K25Ac and NHP10 are synthetically required for growth in the presence of HU. Assays were performed as in Fig. 2C with various HU concentrations.