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. 2011 Jun 13;108(26):10562–10567. doi: 10.1073/pnas.1107559108

Fig. 4.

Fig. 4.

Mice expressing L188Q SPTPC had greater AEC death after i.t. bleomycin. (A and B) TUNEL-stained lung sections from WT (A) and L188Q SFTPC (B) mice at 1 wk after i.t. bleomycin. Arrows point to representative TUNEL+ cells. (Magnification: ×600.) (C) Semiquantitative evaluation of TUNEL+ AECs on lung sections from WT and L188Q SFTPC mice at 1 wk after i.t. bleomycin. (n = 8–11 per group; *P < 0.05 between groups.) (D and E) Western blot analysis (D) and densitometry (E) for caspase-3 using whole-lung lysates from bleomycin-treated WT and L188Q SFTPC mice. β-Actin was used as a loading control. Graphical data represent the ratio of band densities for active caspase-3 and total caspase-3 (WT normalized to value of 1). (n = 5 per group; *P < 0.05 compared with WT.) (F and G) Western blot analysis (F) and densitometry (G) for caspase-12 from lung lysates. Graphical data represent the band density of caspase-12 adjusted for β-actin (WT normalized to value of 1). (n = 5 per group; *P < 0.05 compared with WT.) Graphical data are presented as mean ± SEM.