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. 2011 Jun 13;108(26):10437–10442. doi: 10.1073/pnas.1105197108

Table 1.

Functionalization of Pcl proteins

Reagent Structure(s) Coupling [%] Fig.
PEGs (linear and branched) graphic file with name pnas.1105197108figX1.jpg 50 to > 95 3, 5
Disaccharide graphic file with name pnas.1105197108figX2.jpg > 98 SI Appendix: Fig. S8
DOPE phospholipid graphic file with name pnas.1105197108figX3.jpg > 94 SI Appendix: Fig. S9
Biotin graphic file with name pnas.1105197108figX4.jpg > 98 SI Appendix: Fig. S10
Fluorescein graphic file with name pnas.1105197108figX5.jpg > 88 SI Appendix: Fig. S11
Nitro-phenyl haptens graphic file with name pnas.1105197108figX6.jpg > 93 SI Appendix: Fig. S12
CpG- phosphothioate graphic file with name pnas.1105197108figX7.jpg ∼80 SI Appendix: Fig. S13
PADRE peptide (T-cell epitope) graphic file with name pnas.1105197108figX8.jpg > 83 SI Appendix: Fig. S14

Pcl reagents tested and coupling efficiencies at pH 7.4 and 22 °C. For details and results, see Fig. 3, Fig. 5, and SI Appendix: Figs. S8–S14 as indicated