Figure 3.

Expression of CAB, CHS , and PET E in laf1. Four-day-old seedlings were grown either in the dark under different FR light fluencies (1.5, 3, and 6 μmole/m² sec) or in W light for 18 h. Each lane contained 10 μg of total RNA. CAB, CHS, and PET E were used as probes. (A) Representative Northern blots. The hybridization with the 18S rDNA probe was used as a loading control. (B) Quantitative expression levels of CAB, CHS, and PET E in WT (Ler), laf1, and phyA. Northern blots were quantified with a PhosphoImager, and the expression levels were normalized with respect to the 18S rRNA level.