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The laf1 mutant is specifically disrupted in phyA-dependent gene regulation. Four-day-old seedlings (Ler, phyA, phyB, and laf1) were grown in darkness and then, where indicated, transferred to R (35 μmole/m2 sec) or FR light (3 μmole/m2 sec) for 2 or 18 h. Each lane contained 10 μg of total RNA. CAB, CHS, and XTR7 were used as probes. The 18S rRNA band was used as a loading control.
