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. 2001 Oct 1;15(19):2515–2519. doi: 10.1101/gad.924301

Figure 2.

Figure 2

The use of the waffle vector to generate allelic transgenes that contain DPE- or TATA-dependent reporter genes. (A) Selective excision of the TATA–GFP or DPE–GFP reporter genes from the P-TATA/DPE transposon. The P-TATA/DPE transposon (top) contains both TATA–GFP and DPE–GFP reporter genes. FLP recombinase recognizes the FRT sites and excises the mini-white and TATA–GFP genes. Cre recombinase recognizes the loxP sites and excises the DPE–GFP and mini-white genes. The two resulting sister lines thus contain either DPE–GFP or TATA–GFP at precisely the same genomic position. (B) Sequences of analogous DPE- or TATA-containing core promoters. The segment from the Inr through the DPE is from the Drosophila Antennapedia P2 core promoter, whereas the upstream TATA box is from the adenovirus major late promoter. The two promoters are identical except for 7 bp at the TATA region (−31 to −25) and 7 bp at the DPE region (+28 to +34). The lower case letters denote mutations in the TATA or DPE motifs. (C) In vitro transcription analysis of core promoters containing a DPE, a TATA box, or neither a DPE nor a TATA box.