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The DPE-specific enhancer in the jb23 fly line initiates transcription from the correct +1 start site in vivo. Poly A+ mRNA was purified from embryos of the DPE and TATA sister lines and then subjected to primer extension analysis. There is no detectable specific transcription from the TATA–GFP reporter gene. As a control for the integrity of the poly A+ RNA, transcripts derived from the Drosophila actin 5C gene were analyzed in parallel, as shown at the bottom.
