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. 2001 Apr 1;29(7):1491–1506. doi: 10.1093/nar/29.7.1491

Figure 5.

Figure 5

Relationship amongst enzymes functionally similar to known solitary MTases, Dam and CcrM. Aligned 16S rRNA sequences were obtained from the Ribosomal Database Project (http://www.cme.msu.edu/RDP/html/index.html), and only the part of the alignment common to all was used in constructing the tree. (A) Dendrogram of 16S rRNA sequences from organisms that have been tested for GATC-specific adenine methylation (5) or that possess sequenced GATC-specific MTases of type N6mA(α). The number of restriction endonucleases known to recognize GATC is given followed by the total number of known endonucleases, for all bacteria beyond the indicated branch point. Lines are colored according to taxonomic groups. Tags at the end of branches are green if GATC-specific methylation has been demonstrated, dark green if the strain possesses a GATC-specific MTase and orange if the strain possesses a proven solitary GATC-specific MTase. (B) Dendrogram of all GATC-specific MTases of type N6mA(α) for which sequences are available. The lines are colored as in (A). The source of the sequences are given in the legend to Figure 4, except for Dam from Yersinia pseudotuberculosis (AAG23175), Dam from Serratia marcescens (P45454), Dam from S.typhimurium (P55893), gene from V.cholerae O395 (AAG23174), gene from H.influenzae Rf (P44431), Dam from Neisseria meningitidis BF13 (AAD34292), gene from E.coli retron Ec67 (P21311) and gene from enteric plasmid R27 (AAF69879). (C) Dendrogram of 16S rRNA sequences from organisms that possess sequenced GAnTC-specific MTases. The conventions are analogous to those described in (A). (D) Dendrogram of all GAnTC-specific MTases for which sequences are available. The lines are colored as in (C). The source of the sequences are (reading counterclockwise from Agrobacterium): gene from Agrobacterium tumefaciens C58 (Brad Goodner, personal communication), gene from Sinorhizobium meliloti 1021 (AAB71350), M.BabI (O30570), CcrM (Q45971), gene from Thermoplasma acidophilum DSM1728 (CAC12293), M.CviBI (Q01511), M.CviQVI (AAC03126), M.HhaII (P00473), M.HpyAIV (H64688), M.Hpy99IX (F71827) and M.HinfI (P20590).