Table 3. Proteins similar to those of putative Type I RM systems of Anabaena.
| Targeta |
Proteinb |
Source |
Scorec |
Accession no.d |
| Sequences similar to orf308A Type IB RM proteins | ||||
| HsdM | -. Orf311A | Anabaena | 1 × 10–153 | Genome sitee |
| (271 amino acid C-terminus) | 1. EcoE HsdM | Escherichia | 2 × 10–94 | pir:I41293 |
| 2. EcoA HsdM | Escherichia | 2 × 10–94 | pir:A47200 | |
| 3. StySKI HsdM | Salmonella | 9 × 10–93 | gb:CAA71895 | |
| -. Orf658 | Nostoc | 6 × 10–23 | Genome sitef | |
| -. Orf588 | Nostoc | 1 × 10–4 | Genome sitef | |
| HsdS | -. Orf311A | Anabaena | 3 × 10–90 | Genome sitee |
| (550 amino acid) | 1. CfrA | Citrobacter | 3 × 10–58 | pir:S06097 |
| 2. EcoE | Escherichia | 2 × 10–47 | pir:P19705 | |
| 3. EcoA | Escherichia | 2 × 10–46 | pir:P19704 | |
| -. Orf658 | Nostoc | 4 × 10–8 | Genome sitef | |
| HsdR | -. Orf308B | Anabaena | <10–200 | Genome sitee |
| (430 amino acid C-terminus) | 1. EcoA | Escherichia | 1 × 10–141 | pir:I41291 |
| 2. EcoE | Escherichia | 1 × 10–140 | pir:I41292 | |
| 3. StySKI | Salmonella | 2 × 10–53 | gb:CAA71894 | |
| -. Orf658 | Nostoc | 7 × 10–8 | Genome sitef | |
| Sequences similar to Orf308B/311A Type IB RM proteins | ||||
| HsdM | 1. EcoE HsdM | Escherichia | 1 × 10–101 | pir:I41293 |
| (296 amino acid C-terminus) | 2. EcoA HsdM | Escherichia | 1 × 10–101 | pir:A47200 |
| 3. StySKI HsdM | Salmonella | 1 × 10–100 | gb:CAA71895 | |
| HsdS | 1. EcoA | Escherichia | 4 × 10–81 | sp:P19704 |
| (542 amino acid) | 2. StySKI | Salmonella | 1 × 10–40 | gb:CAA71896 |
| 3. CfrA | Citrobacter | 7 × 10–35 | gb:CAA35604 | |
| 4. EcoE | Escherichia | 8 × 10–30 | sp:P19705 | |
| HsdR | 1. EcoA | Escherichia | <10–200 | pir:I41291 |
| (776 amino acid) | 2. EcoE | Escherichia | <10–200 | pir:I41292 |
| 3. StySKI | Salmonella | 3 × 10–53 | gb:CAA71894 | |
| Protein sequences similar to Orf260 Type IC RM protein | ||||
| HsdM | 1. LldI | Lactococcus | 1 × 10–120 | pir:T09460 |
| (537 amino acid) | 7. HsdM | Helicobacter | 1 × 10–93 | pir:E71886 |
| -. Orf339A | Anabaena | 1 × 10–67 | Genome sitee | |
| -. Orf376B | Anabaena | 4 × 10–47 | Genome sitee | |
| -. Orf641 | Nostoc | 4 × 10–21 | Genome sitef | |
| HsdR | 1. HsdR | Helicobacter | 1 × 10–127 | pir:B71890 |
| (933 amino acid) | -. Orf376B | Anabaena | 1 × 10–25 | Genome sitee |
| -. Orf641 | Nostoc | 3 × 10–10 | Genome sitef | |
| Sequences similar to Orf339A Type IC RM protein | ||||
| HsdM | 1. HsdM | Helicobacter | 1 × 10–180 | pir:C71810 |
| (803 amino acid) | -. Orf376B | Anabaena | 6 × 10–43 | Genome sitee |
| -. Orf641 | Nostoc | 8 × 10–19 | Genome sitef | |
| HsdS | 1. HsdS | Methanococcus | 8 × 10–22 | pir:B64316 |
| (425 amino acid) | -. Orf641 | Nostoc | 3 × 10–3 | Genome sitef |
| HsdR | 1. HsdR(179) | Klebsiella | 6 × 10–65 | pir:T30818 |
| (179 amino acid N-terminus) | -. Orf376B | Anabaena | 2 × 10–4 | Genome sitee |
| Sequences similar to Orf376B Type ID RM protein | ||||
| HsdM | 1. Cj1553c | Campylobacter | 1 × 10–171 | gb:CAB73544 |
| (527 amino acid) | 2. HsdM | Pasteurella | 1 × 10–135 | gb:AAC44666 |
| -. Orf641 | Nostoc | 4 × 10–20 | Genome sitef | |
| HsdS | 1. Cj1551c | Campylobacter | 6 × 10–29 | gb:CAB73967 |
| (390 amino acid) | 2. MJECL41 | Methanobacter | 5 × 10–15 | pir:H64514 |
| HsdR | 1. Cj1549c | Campylobacter | <10–200 | gb:CAB73965 |
| (1011 amino acid) | 2. HI1285 | Haemophilus | <10–200 | pir:F64114 |
| 3. HsdR | Pasteurella | 1 × 10–198 | pir:JC5216 | |
| -. Orf641 | Nostoc | 6 × 10–8 | Genome sitef | |
aProtein sequence from Anabaena used in BLAST search (26) of the combined GenBank, PDB, SwissProt, PIR and PRF databases, followed by length of sequence in amino acid residues. Partial sequences were used when full sequences were unavailable. Cyanobacterial sequences are shown in bold.
bProteins found by BLAST search, ranked by score. Scores of proteins not found in combined databases were obtained by a pairwise BLAST comparison.
cScore represents expected number of proteins at least as similar to target within the combined databases.
dgb, GenBank; sp, SwissProt; pir, Protein Information Resource.
eSequence obtained from the Anabaena web site (http://www.kazusa.or.jp/cyano/anabaena/), last accessed June 2000.
fSequences obtained from the N.punctiforme web site (http://spider.jgi-psf.org/JGI_microbial/html/nostoc_homepage.html), last accessed June 2000.