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. 2001 Nov 1;15(21):2865–2876. doi: 10.1101/gad.934301

Figure 5.

Figure 5

Evidence of massive cell death and decreased expression of p57 mRNA in mutant growth plate chondrocytes. (a) in situ hybridization analysis with collagen type II cRNA on histological sections from newborn wild-type and mutant tibias and rib cages, respectively; the darkfields of the proximal epiphyses and of the rib cages for both wild-type and mutant specimens are shown. (b) In situ hybridization analysis with collagen type X cRNA on histological sections from E15.5 wild-type and null tibias and newborn rib cages, respectively; the darkfields of the proximal epiphyses and of the rib cages for both wild-type and mutant specimens are shown. (c) In situ hybridization analysis with p57 cRNA on histological sections from E18.5 wild-type and mutant tibias; the darkfields for both wild-type and mutant specimens are shown. (d) Fluorescence detection (FITC) of TUNEL positive cells in the proximal epiphyses of wild-type and mutant newborn tibias; in comparison to the wild-type specimen, numerous TUNEL-positive cells are clearly evident in the mutant.