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. 2001 Nov 1;15(21):2865–2876. doi: 10.1101/gad.934301

Figure 7.

Figure 7

Evidence for HIF-1α-dependent and -independent regulation of VEGF expression, increase of EF-5 binding, and ectopic angiogenesis in the mutant growth plate. (a,b) In situ hybridization analysis with VEGF cRNA on histological sections E15.5 from wild-type and mutant tibias and fibulas; the darkfields for both wild-type and mutant specimens are shown (exposure to photoemulsion = 14 d). (c,d) In situ hybridization analysis with VEGF cRNA on histological sections from E15.5 mutant femur; brightfield and darkfield are shown, respectively (exposure to photoemulsion = 24 d) . (e,f) Immunofluorescence detection of EF-5 in proximal epiphysis of E18.5 wild-type and mutant tibia, respectively. (g–j) H&E staining of histological sections of proximal epiphyses of mutant newborn tibias; square and circles are drawn around areas of ectopic angiogenesis; h is a higher magnification image of g.