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. 2011 Jun 30;6(6):e20930. doi: 10.1371/journal.pone.0020930

Figure 3. Expression and alignment of the AP3 genes in wild-type and mutant B. napus.

Figure 3

(A) Expression of AP3 genes in wild-type and mutants of B. napus; (B) DNA amplification of WTn and Apt with the 24-bp sequence specific primers 24-F and 24-R; (C) DNA amplification of Apt, AMSb and AMSa with AP3-F and AP3-R primers; (D) Nucleic acid alignment of BnaA.AP3.b and BnaC.AP3.b of B. napus (only the SNPs are shown). BnaC.AP3.b-Mu is the BnaC.AP3.b gene with a G–T single-nucleotide mutation (shown by an arrow); (E) Nucleic acid alignment of BnaA.AP3.a and BnaC.AP3.a of B. napus (only the SNPs are shown); D5 is the BnaC.AP3.a gene with an 86-bp foreign insertion in its second exon (shown by an arrow).