Table 1.
Suppression of mms4 and mus81 sporulation defects
| Genotype
|
Sporulation efficiencya (%)
|
Spore viabilityb (%)
|
|---|---|---|
| wild type | 35 | 83 |
| spo11 | 4.9 | ND |
| spo13 | 19 | ND |
| spo11 spo13 | 20 | 77 |
| mms4 | 0.43 | 6 |
| mms4 spo11 | 3.1 | ND |
| mms4 spo13 | 1.5 | ND |
| mms4 spo11 spo13 | 22 | 73 |
| mus81 | 0.52 | 12 |
| mus81 spo11 | 4.8 | ND |
| mus81 spo13 | 1.2 | ND |
| mus81 spo11 spo13 | 21 | 83 |
a
For wild-type cells, sporulation refers to four-spored asci, and for all others it refers to two-spored asci. Sporulation efficiency was determined following 6 d in sporulation medium at 30°C. The value presented for each genotype is an average of two strains (at least 500 cells were counted per strain).
b
Viability was determined by the ability of micro-dissected spores to form colonies. At least 50 spores were examined for each genotype.