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. 2011 Jun 30;6(6):e21484. doi: 10.1371/journal.pone.0021484

Figure 3. PAR-1 neutralization reduces GrK-induced IL-6, IL-8 and MCP-1 production.

Figure 3

Cells were incubated with the PAR-1 neutralizing antibody (ATAP-2; 5 µg/ml) PAR-2 neutralizing antibody (Sam11L; 25 µg/ml) or mouse isotype IgG control antibody for 30 min prior to incubation with GrK (200 nM) for 24 h. Supernatants from cell cultures were collected and analyzed for IL-6, IL-8 and MCP-1 production by ELISA. Cell counts were used to normalized to cell numbers. Data are expressed as % of media control (DMEM+0.0001% NaN3) ± SEM from three separate experiments run in triplicate. + p<0.05 when compared to media control; ** p<0.05 when compared to GrK (200 nM) treatment.