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. 2011 Jun 30;6(6):e21542. doi: 10.1371/journal.pone.0021542

Figure 1. h-CaD and ERK 1/2 expression in NIL and L term human myometrium.

Figure 1

NIL and L myometrial protein extracts were separated by SDS-PAGE and western transferred onto nitrocellulose membrane. Membranes were probed with antibodies against phospho-h-CaD (1∶2000), total h-CaD (1∶2000), phospho-ERK 1/2 (1∶2000), total ERK 1/2 (1∶2000) and α-SMA (1∶10000). A total 8×NIL and 8×L samples were analysed. Representative image demonstrates detection of these proteins on a single blot containing 4×NIL and 4×L myometrial samples, as well as a calibrator sample that was included to allow densitometric comparison across separate blots. Mr = relative molecular mass×1000.