Figure 1. Developmentally regulated A. gambiae sex-biased genes.

(A) Number of female- (pink) and male- (blue) biased genes transcribed at the larval, pupal and adult stages. The bars are split in light and dark colours to arrange sex-biased genes according to two different thresholds of male:female log₂ expression ratios +/− 0.8 and +/− 1.6 respectively. (B) Venn diagram showing the relationship between sex-biased transcription and development. Larval stages were pooled together to simplify their visualization. The relative percentage of sex-biased genes respect to a total of 1544 genes with transcription values at all stages examined is shown in parenthesis. (C) Variance of gene expression across different developmental stages at increasing male:female expression ratios. The average male:female expression ratio of each gene at all stage examined is plotted against the standard deviation of its expression. The male-biased expression is the most relevant turnover of sex-linked expression across mosquito development though at the adult stage female-biased genes are more numerous than the male-biased ones. See also Figure S1 and Tables S1, S2, S3.