Table 2. RNAi mediated targeting of selected sex-biased genes selectively transcribed either in the testis only or in both the testes and the ovaries.
Gonad development anomalies | |||||
Gene ID | Protein domain ID & Description | N. embryos injected | N. mosquitoes eclosed | Male | Female |
Testis –transcribed genes | |||||
AGAP001388 | IPR001275: DM DNA-binding | 1089 | 87 | 0/41 | 0/46 |
AGAP010341 | IPR008271: Serine/threonine protein kinase, active site | 878 | 73 | 0/39 | 0/34 |
AGAP001165 | IPR007087: Zinc finger, C2H2-type | 810 | 112 | 0/59 | 0/53 |
AGAP007664 | IPR000956: Stathmin | 840 | 34 | 0/18 | 0/16 |
AGAP008765 | IPR008271: Serine/threonine protein kinase, active site | 1637 | 111 | 1*/54 | 0/57 |
AGAP008976 | IPR002373: cAMP/cGMP-dependent protein kinase | 857 | 58 | 0/30 | 0/28 |
AGAP008341 | IPR008271: Serine/threonine protein kinase, active site | 876 | 48 | 0/23 | 0/25 |
Testis and ovaries transcribed genes | |||||
AGAP006241 | IPR000990: Innexin | 1621 | 126 | 27/60 | 34/66 |
AGAP006449 | Pfam 08598: SDS3 domain | 1583 | 144 | 7/73 | 6/71 |
Control | |||||
LacZ | 1214 | 87 | 0/42 | 0/45 |
*A single individual showed one normal testis on one side and a duplicated testis on the other side.