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. 2011 Jun 30;6(6):e21740. doi: 10.1371/journal.pone.0021740

Table 2. Mutations in genomes of mouse-adapted variants obtained after 12 and 20 mouse-lung passages relative to HK-wt.

		HKMA12 clones and mutations						HKMA20 clones and mutations
gene	HK-wt^a	12^b	12A	12B	12C	12D	12E	20^b	20A	20B	20C^a	20D	20E	positive selection
PB2	D701	N	N	N	N	N	N	N	N	N	N	N	N	yes
	D740	•	•	•	•	•	•	•	N	•	•	•	•	nd
PB1	R190	•	•	•	•	•	•	K	•	•	•	•	•	nd
	K578	•	T	•	•	T	•	T	•	•	•	T	•	yes
PA	D27	G	•	•	G	•	•	•	•	•	•	•	•	yes
	Q556	•	•	•	•	•	R	R	R	R	R	R	R	yes
	E610	•	•	G	•	•	•	•	•	•	•	•	•	nd
	K673	•	•	R	•	•	•	•	•	•	•	•	•	nd
HA^c	D2¹	•	Y	•	•	•	•	•	•	•	•	•	•	nd
	P162¹	•	•	L	•	•	•	•	•	•	•	•	•	nd
	G218¹	W	•	•	W	W	W	W	W	W	W	W	W	yes
	N246¹	•	D	•	•	•	•	•	•	•	•	•	•	nd
	N154²	•	•	•	•	•	•	•	•	•	•	S	•	yes
	T156²	N	•	•	N	N	•	N	N	N	N	•	N	yes
	D158²	•	•	N	•	•	•	•	•	•	•	•	•	nd
NP	D34	N	N	N	N	N	N	N	N	N	N	N	N	yes
	D480	•	•	•	•	•	•	•	•	N	N	N	•	yes
NA	A110	•	•	•	•	•	•	•	•	•	•	V	•	nd
	P468	H	H	H	H	•	•	H	•	H	H	•	H	yes
M1^b	D232	•	•	•	•	•	•	N	N	•	•	•	•	yes
M2^b	D44	•	•	•	•	•	•	•	•	N	N	.	N	yes
NS1^a	V23	•	•	•	•	A	•	A	A	•	•	•	•	yes
	F103	•	•	•	•	•	•	•	•	L	L	L	•	yes
NEP^a	K88	•	•	•	•	•	•	•	•	R	•	•	•	nd

^a

previously independently sequenced in [59].

^b

partially independently sequenced as reported in [59].

^c

mutation found in multiple clones is indicated (yes) versus not-detected (nd).

^d

independently sequenced in [10].

^e

HA mutations in the HA1 and HA2 subunits are indicated with superscripts 1 and 2 respectively.

^f

identity to HK-wt amino acids is indicated by dots.

^g

same loss of glycosylation as HA T156²N.