Table 1. Phenotypic analysis of monocytes and MDDC.

Cell type	CD14	CD1a	CD11b	CD40	CD80
Monocytes	95±1.3	0.22±0.17	66±7.2	0±0	0.12±0.01
MDDC	13±3.4	31±6.2	99±0.11	8.0±3.3	8.9±2.9
p-value	<0.001	<0.05	<0.05	0.074	<0.05

PBMCs were isolated from fresh donor blood buffy coats. After an aggregation step, the cells were cultured with or without 25 ng/ml IL-4 and 50 ng/ml GM-CSF for 5 days to derive MDDC or monocytes, respectively. Expression of several cell surface markers was analyzed flow cytometrically with specific PE-labeled mAbs. Data represent mean % positive cells ± standard error of the mean (SEM) from 5 different donors. The expression level of each marker was compared between monocytes and MDDC and the corresponding p-value was calculated with a paired t-test.