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. 2011 Jun 30;6(6):e21767. doi: 10.1371/journal.pone.0021767

Figure 1. Phosphatase activity is associated with the TLRR complex immunoprecipitated from adult testis lysate.

Figure 1

TLRR complex phosphatase activity was measured using the EnzChek® Phophatase Assay Kit (Invitrogen) as described in Materials and Methods. Either TLRR antibody or Normal Rabbit IgG (NRIgG) as negative control were linked to Sepharose beads and incubated with 1 mg (25 µl of 40 µg/µl) CD-1 adult mouse testis lysate. Proteins bound to beads were eluted and added to fluorescence substrate and resultant fluorescence was measured after 60 min. The results are significant at p = 0.01.