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. 2011 Jun 30;6(6):e21767. doi: 10.1371/journal.pone.0021767

Figure 2. TLRR-associated phosphatase activity varies with developmental stage of the testis.

Figure 2

(A) Testis lysate from day 7, 14, 21, 35, or 56 mice was incubated with Sepharose beads linked to TLRR antibody. Protein phosphatase activity of bound proteins was measured as described in Figure 1 using the Enzchek® assay for total phosphatase activity and the results are displayed relative to the activity at 56 days (set as 100%). Protein phosphatase activity at day 7 and day 21 are significantly different at p = 0.05. (B) Testis lysate from day 7, 14, 21, 35, or 56 mice was immunoprecipitated with TLRR antibody beads and the PP1-specific phosphatase activity in bound proteins was measured with Rediplate 96 EnzChek® Serine/Threonine Phosphatase Assay kit (Invitrogen). Fluorescence was measured at 460 nm. PP1 activity is significantly different between day 7 and day 21 (asterisks) and between day 21 and day 35 (deltas). Differences are significant at p = 0.05.