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2 mg testis lysate from animals at the indicated days after birth was immunoprecipitated with either the pan-PP1 antibody FL-18 (A) or an antibody specific to the gamma 2 isoform (B). Bound proteins were separated by SDS-PAGE, transferred to membrane and probed with the corresponding antibody or an antibody to TLRR. In the negative control (-C) normal rabbit IgG was used in place of the PP1 antibody.
