Figure 6. TLRR, kinesin-1B and tubulin are phosphorylated during the first round of spermatogenesis in the developing testis.

2 mg testis lysate from animals at the indicated days after birth was immunoprecipitated with a mixture of anti-phosphoserine antibodies as described in Materials and Methods. Immunoprecipitated proteins were separated by SDS-PAGE, transferred to membrane and blotted with antibodies specific to the proteins indicated to the right. –C indicates proteins immunoprecipitated with normal rabbit IgG.