Table 1. Fold reduction in chromosomal transformation frequency1 in ssbB −, ssbBΔ7, and ssbBΔ27 cells2.
Experiment3 | ssbB − | ssbBΔ7 | ssbBΔ27 |
I | 13.5±5.44 | 3.4±1.4 | -5 |
II | 4.8±1.9 | 2.1±0.8 | - |
III | 9.5±2.3 | 2.2±0.5 | - |
IV | 6.0±2.3 | 3.6±1.6 | 5.7±0.6 |
V | 6.9±3.1 | - | - |
Competent cells were transformed with R304 donor DNA and transformants resistant to streptomycin (SmR; rpsL41 point mutation) were scored.
Absolute transformation frequencies are shown in Table S1.
Fold reduction in SmR transformants relative to the wildtype parent strain indicated in footnote 3.
Transformations have been carried out in parallel for all strains in the same experiment: strains R1818, R2646 and R2647 in experiments I–III; strains R1501, R1988, R2081 and R2082 in experiment IV; and strains R1501 and R1988 in experiment V.
Ratio errors calculated as indicated in Table S1.
Not done.