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. 2011 Jun 30;7(6):e1002109. doi: 10.1371/journal.ppat.1002109

Figure 5. HSV-2 infection increases ALDH1A1 expression in moDCs.

Figure 5

A) Expression of ALDH1A1, ALDH1A2, and ALDH1A3 RNA in immature moDCs and PBMCs by RT-PCR. Results shown are representative of data from 2 different donors. A negative control (−) without DNA was included to control for contamination. GAPDH was amplified in each sample as an internal PCR and loading control. B) The fold changes (mean ± SEM, 13 independent experiments) in ALDH1A1 mRNA of LIVE- and UV-HSV-2-treated moDCs (24 h post treatment) are shown relative to mock-treated moDCs (set as 1). C) The fold changes (mean ± SEM, 5 independent experiments) in the percentage of ALDEFLUOR positive (ALDH enzymatic activity) moDCs treated with LIVE or UV-HSV-2 (24 h post treatment) are shown relative to mock-treated moDCs (set as 1). D) Gating strategy for ALDH enzymatic activity in mock-, UV-HSV-2-, or Live HSV-2-treated moDCs (summarized in panel C). One representative of 5 independent experiments is shown. The numbers indicate the percentage of ALDH+ cells. (*p<0.05, **<0.01, ***p≤0.001).