Figure 2. Extramedullary B lymphopoiesis in spleen during T. brucei infection.

(A) Spleens cells from non-infected mice and mice infected with T. brucei for 6–30 days were stained for surface markers commonly used to define developing B cells as described in table 1 and analyzed using FACS. Data are represented as mean of three mice per group ± SEM, two independent repeat experiments were performed and statistics are compared to uninfected controls (*) p<0,05, (**) p<0,01, (***) p<0,001. (B) Pre-pro-B and Pro-B cells were detected as (Lin⁻B220⁺IgM⁺CD43^hi) and respectively AA4.1⁺CD19⁻ or AA4.1⁺CD19⁺ in uninfected mice (left panel) versus infected mice on day 10 p.i. (right panel).