Figure 3. Depletion of transitional B cells in spleen during T. brucei infection.

(A) Spleens cells from non-infected mice and mice infected with T. brucei for 6–30 days were stained for surface markers commonly used to define transitional T1, T2 and T3 B cells and analyzed using FACS. Data are presented as mean of three mice per group ± SEM, three independent repeat experiments were performed and statistics are compared to uninfected controls (**) p<0,01. (B) Transitional T1, T2 and T3 B cells were detected as (AA4.1⁺B220⁺) and respectively IgM⁺CD23⁻, IgM⁺CD23⁺ and IgM^loCD23⁺ in uninfected mice (left FACS panel) and infected mice on day 6 p.i. (middle FACS panel) and day 20 p.i. (right FACS panel).