Figure 8. In vivo anti-FasL treatment of T. brucei infected mice.

Mice infected with T. brucei were treated with 100 µg of anti-FasL antibody on days 4, 5 and 6 of infection and on day 7 of infection flow cytometric analysis was performed. (A) FACS plot from transitional T1, T2 and T3 B cells, detected as (AA4.1⁺B220⁺) and respectively IgM⁺CD23⁻, IgM⁺CD23⁺ and IgM^loCD23⁺ in infected but untreated control mice (left FACS panel), and infected anti-FasL treated mice (right FACS panel). (B) Representative histogram of percentage of apoptotic cells within T1 (left) and T2 (right) transitional B cell populations in uninfected controls (gray area) versus uninfected but treated mice (thin black line) and infected treated mice (thick black line). Data are represented as mean of five mice per group ± SEM.