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. 2011 Jun 30;7(6):e1002089. doi: 10.1371/journal.ppat.1002089

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Bockstal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Total number of viable (or poly caspases-negative) transitional B (T1 and T2) cells left after 20 h of co-culture of total spleen cells from uninfected mice, or mice on day 5 of infection, with live bloodstream form trypanosomes or with medium only as control, or total spleen cells from uninfected mice and trypanosomes separated by a 0,4 µm polycarbonate transmembrane.. Data are presented as mean of three mice per group ± SEM and three independent repeat experiments were performed (**) p<0,01, (***) p<0,001. (B) Total number of viable transitional B (T1 and T2) cells left after 20 h of co-culture of total spleen cells from mice on day 5 of infection with trypanosome lysate, T. brucei soluble VSG (sVSG), live bloodstream form trypanosomes, live trypanosomes pre-incubated with VSG-specific nanobodies or only medium as control. Data are presented as mean of three mice per group ± SEM and two independent repeat experiments were performed (**) p<0,01, (***) p<0,001.