Table 1. Differentiation antigen phenotypes of developing and mature B2 B cells.
Suface marker phenotype | |
HSC/LMPP | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220− , IL-7r−, ckit+ |
CLP | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220−, ckit−, AA4.1+, IL-7r+ |
pre-pro-B | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220+, AA4.1+, IgM−, CD19−, CD43hi |
pro-B | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220+, AA4.1+, IgM−, CD19+, CD43hi |
pre-B | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220+, AA4.1+, IgM−, CD19+, CD43lo/− |
immature B | Lin− (Ter119, CD3ε, CD11b, Gr1, NK1.1), B220+, AA4.1+, IgM+, CD19+, CD43lo/− |
T1 transitional | B220+, AA4.1+, IgMhi, CD23− |
T2 transitional | B220+, AA4.1+, IgMhi, CD23+ |
T3 transitional | B220+, AA4.1+, IgMlo, CD23+ |
MZB | B220+, AA4.1−, CD1dhi |
FoB | B220+, AA4.1−, CD1dlo |
B lymphocyte subsets (left column) were identified and defined based on flow cytometric analysis using surface expression of specific antigens (right column).