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. Author manuscript; available in PMC: 2012 Jun 26.
Published in final edited form as: J Med Chem. 2011 May 26;54(12):4057–4066. doi: 10.1021/jm200028h

Figure 5. Pharmacogenomic effect of HB in vivo.

Figure 5

(A) RNA was extracted from pooled (n=5) gastrocnemius muscle samples of control and HB-treated animals (60 mg/kg for 24 d) and analyzed using rat insulin signaling pathway PCR array to measure relative gene expression levels for 84 genes. Central black line indicates fold changes ((2^(-ΔCt)) of 1, while the pink lines indicate the 4 fold-change in gene expression threshold. (B) Results for Igf2 gene expression and (C) a set of the myogenic transcriptional factors that modulate muscle growth and differentiation were further confirmed by conventional RT-PCR on individual muscle samples (n=5) from control and HD-treated animals. Results are expressed as the mean ± SEM of determinations performed in duplicate (* P<0.05 when compared with control by Student t test).