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. 2011 Jun 30;7(6):e1002133. doi: 10.1371/journal.ppat.1002133

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(A) Strategy for construction of the rrp1 mutant and the repaired strain (rrp1^com). Arrows indicate the approximate positions of the oligonucleotide primers used for PCR analysis. (B) PCR analysis of strains. The specific primer pairs used in PCR are indicated above lanes. Lane W, wild-type (5A4NP1); lane M, the rrp1 mutant; lane C, rrp1^com. (C) Western blot analysis of whole-cell lysates of WT, rrp1, and rrp1^com spirochetes probed with α-Rrp1 and α-FlaB monoclonal antibodies.