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. 2011 Jun 30;7(6):e1002133. doi: 10.1371/journal.ppat.1002133

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Unfed I. scapularis nymphs were microinjected with wild-type (5A4NP1), rrp1, or rrp1^com spirochetes and then fed on naïve mice. The engorged nymphs were collected after repletion and subjected to IFA analysis using fluorescein isothiocyanate-labeled anti-B. burgdorferi antibody. Ten ticks were examined in each group and a representative image for each group of ticks is shown in this figure.