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. 2011 Jun 30;7(6):e1002133. doi: 10.1371/journal.ppat.1002133

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Relative transcript levels of the glycerol operon glp (bb0240-bb0243) in wild-type, rrp1, and rrp1^com by real-time RT-PCR. RNA was isolated from late logarithmic phase cultures grown at 35°C in standard BSK-II medium. Values represent the average copy number for each gene (± standard deviation) normalized per 1000 copies of flaB. (B–E) growth curves of wild-type, rrp1, rrp1^com or rrp1/flaBp-glp at 23°C (D, E) or 35°C (B, C) in standard BSK-II medium (B, D) or BSK-glycerol medium (C, E). The initial cell density was 3×10⁵ cells/ml for each strain. Spirochetes were enumerated under dark-field microscopy. Data presented here is from one representative experiment with three independent cultures. Each data point was the average of data from three independent cultures. *, P<0.05.