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. 2011 Jun 30;7(6):e1002133. doi: 10.1371/journal.ppat.1002133

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Wild-type B. burgdorferi strain B31 5A4NP1 was grown at 35°C in either standard BSKII or BSK-glycerol medium. Cells were harvested at late logarithmic phase. (A) qRT-PCR. RNAs were extracted and subjected to real-time RT-PCR analyses for rrp1, bb0240, bb0241, bb0242, bb0243, rpoS, ospC, and flaB. Levels of expression of each gene were normalized with the level of flaB expression in each sample. Relative fold change of gene expression between the two growth conditions were reported (with levels of expression of each gene in standard BSKII media as values of 1). **, p<0.01. (B) immunoblot against Rrp1, FlaB, or OspC. Lane 1, spirochetes cultivated in BSK-II medium. Lane 2, spirochetes cultivated in BSK-glycerol medium. Upon normalization against FlaB, Rrp1 level is 1.7 fold higher in BSK-glycerol medium than that in standard medium.