Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effects of mutating individual phosphorylation sites on 4E-BP1 phosphorylation. HA-tagged 4E-BP1 proteins were expressed in HEK 293T cells. At 48 h after transfection, lysates were prepared and analysed by two-dimensional IEF/SDS-PAGE, as above. Western blots were prepared using an antibody directed against the HA tag.
