Figure 2.

Localization of HIF-1α residues interacting with FIH-1. (A) GST and GST–FIH-1 fusion proteins were expressed in E. coli, purified, incubated with ³⁵S-labeled in vitro-translated HIF-1α, captured on glutathione–Sepharose beads, and analyzed by SDS-PAGE and autoradiography. (B–D) GST-fusion proteins containing the indicated HIF-1α residues at their C terminus were incubated with ³⁵S-labeled in vitro-translated FIH-1, captured on glutathione–Sepharose beads, and analyzed as described above. (0) GST only.