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. 2001 Oct 15;15(20):2675–2686. doi: 10.1101/gad.924501

Figure 7.

Figure 7

Figure 7

Figure 7

Interaction of VHL and FIH-1 with histone deacetylases. (A) GST and GST–HDAC fusion proteins were incubated with 35S-labeled FLAG–VHL (top), HA–FIH-1 (middle), or HIF-1α (bottom), captured on glutathione–Sepharose beads, and analyzed by SDS-PAGE and autoradiography. (B) GST–HDAC fusion proteins were incubated with 35S-labeled FLAG–VHL truncated at its C terminus as indicated and analyzed as described above. (C) GST–HDAC fusion proteins were incubated with 35S-labeled FLAG–VHL and/or 35S-labeled HIF-1α.